|
KMID : 1377020200170010033
|
|
Tissue Engineering and Regenerative Medicine
2020 Volume.17 No. 1 p.33 ~ p.44
|
|
|
Enhanced Efficacy of Immunization with a Foot-and-Mouth Disease Multi-Epitope Subunit Vaccine Using Mannan-Decorated Inulin Microparticles
|
|
Yoon So-Yeon
Kang Sang-Kee
Lee Ho-Bin
Oh Seo-Ho
Kim Whee-Soo
Li Hui-Shan
Bok Jin-Duck
Cho Chong-Su
Choi Yun-Jaie
|
|
|
Abstract
|
|
|
BACKGROUND: Despite the many advantages of recombinant subunit vaccines, they have critical weaknesses that include a low efficacy for promoting cellular and humoral immune responses against antigens because of their poor immunogenicity, and a rapidly cleared properties as a result of proteolytic enzymes in the body. To circumvent these problems, we developed mannan-decorated inulin acetate microparticles (M-IA MPs) that functioned as carriers and adjuvants for immunization with the recombinant foot-and-mouth disease multi-epitope subunit vaccine (M5BT).
METHODS: The M5BT-loaded M-IA MPs were obtained by a double-emulsion solvent-evaporation method. Their properties including morphology, size and release ability were determined by field emission scanning electron microscope, dynamic light-scattering spectrophotometer and spectrophotometer. To assess the immunization efficacy of the MPs, mice were immunized with MPs and their sera were analyzed by ELISA.
RESULTS: The M-IA MPs obtained by a double-emulsion solvent-evaporation method were spherical and approximately 2?3 μm, and M5BT was encapsulated in the M-IA MPs. The M5BT-loaded M-IA MPs showed higher antigen-specific IgG, IgG1, IgG2a and anti-FMDV antibodies than the M5BT-loaded IA MPs and the Freund’s adjuvant as a control.
CONCLUSION: The M-IA MPs showed a powerful and multifunctional polymeric system that combined two toll-like receptor agonists compared to the conventional adjuvant.
|
|
|
KEYWORD
|
|
|
Inulin acetate, Microparticle, Mannan, Food-and-mouth disease, Subunit vaccine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
   
|